Rapid plasma membrane reorganisation and endocytosis in HER2 breast cancer cells incubated with trastuzumab decorated polymer nanoparticles

Key Points

• To investigate how trastuzumab-decorated nanoparticles affect the plasma membrane of HER2 breast cancer cells.
• Creation of PLGA nanoparticles decorated with trastuzumab.
• Live imaging using resonant scanning confocal microscopy.
• Scanning electron microscopy (SEM) to observe membrane changes.
• Trastuzumab binding leads to HER receptor clustering on cancer cell surfaces.
• Rapid reorganization of the plasma membrane observed post-incubation.
• Structural changes in the membrane confirmed by SEM.