Protocol for combining BMP, MEK, WNT inhibition and iNGN2 to enable rapid neuronal differentiation and regional patterning from hiPSCs

Key Points

• This research aims to develop a protocol for effective neuronal differentiation and regional patterning from hiPSCs using specific inhibitors and iNGN2.
• Utilized BMP, MEK, and WNT inhibition for pre-differentiation of hiPSCs.
• Induced neuronal differentiation through overexpression of Neurogenin 2 (iNGN2).
• Outlined procedures for neural progenitor replating and maturation of specific neuron types.
• Successful induction of telencephalic neurons from hiPSCs following the combined inhibition protocol.
• Reduction of mixed neuron populations achieved through pre-differentiation.
• Protocols validated for specific neuron patterning including motor neurons and sensory neurons.