Novel H2 colourimetric indicator for screening the activity of H2-generating bacteria and measuring their total viable count (TVC)

Key Points

• To develop a colourimetric H2 indicator for screening H2-generating bacteria and assessing total viable count (TVC).
• Created colourimetric H2 indicator using methylene blue and colloidal Pt particles.
• Encapsulated the mixture in a polymer film for stability.
• Laminated between thin sheets for practical application.
• The indicator successfully detects H2 production from bacteria.
• Correlates well with total viable count (TVC) measurements.
• Provides a simple and effective screening method for H2-generating bacteria.