An Integrated Genotyping Strategy for α/β-Thalassemia: Based on the Analysis of the Coding Sequences and Expression Levels of HBA2, HBA1, and HBB in Peripheral Blood mRNA.

Key Points

• The study aims to develop a strategy for genotyping α/β-thalassemia through the analysis of mRNA.
• Analysis of coding sequences of HBA2, HBA1, HBB genes
• Detection of coding-region mutations
• Prediction of non-coding-region mutations
• Assessment of gross deletions
• Utilization of peripheral blood mRNA samples
• High coverage of detected mutations
• Low operational cost
• Simple procedure for analysis
• Effective prediction of non-coding mutations