A fully automated novel solid-phase extraction method for siRNA drug extraction and quantitation across multiple tissues using LC-MS.

Key Points

• The aim is to present a fully automated method for extracting and quantifying siRNA from various tissues.
• Developed a one-step solid-phase extraction (SPE) method.
• Reduced the need for tissue volume in analyses.
• Utilized surrogate matrix calibration for sample analysis.
• Automated the process to simplify sample preparation.
• Significantly decreased analytical time for tissue biodistribution studies.
• Improved efficiency in sample handling and preparation workloads.
• Maintained adherence to the principles of the 3Rs (Reduce, Replace, Refine).