Ultrasensitive duplex lateral flow immunoassay for enhanced detection of viral antigens using hybridization chain reaction amplification

Key Points

• Viral antigens are detected with greater sensitivity using hybridization chain reaction amplification—indicating advancements in diagnostics.
• The method shows a significant increase in sensitivity, achieving a detection limit of 1.2 fg/mL for viral antigens.
• Assessment using duplex lateral flow immunoassay techniques enhances the reliability and usability of rapid tests.
• This method may enable faster and more accurate detection of viral infections, supporting public health efforts.