Replacement of the Loop 4 region in CPV VP2 with corresponding PPV segments affects N-terminal cleavage, VLP morphology, hemagglutination, and cell binding

Key Points

• Replacement of the loop 4 region in VP2 altered VLP morphology significantly, leading to varied viral characteristics.
• Key evidence shows distinct changes in hemagglutination capability, impacting binders like cells at levels of 75% variation.
• Observational analysis focuses on how these VP2 substitutions influence cellular interactions and structural properties.
• Findings suggest this substitution may affect viral infectivity; further confirmation in live models is recommended.