Fusarium wilt, caused by Fusarium oxysporum ( F. oxysporum ), poses a significant threat to global crop production. Conventional fungicides such as carbendazim (CBZ) encounter substantial challenges, including single-target resistance, poor environmental stability, an effective utilization rate of less than 0.1 %, and biosafety concerns. To overcome these limitations and address the core bottlenecks of existing nanopesticides, we developed a novel intelligent nanopesticide (CBZ@Se-CS, CSC) through a straightforward non-covalent self-assembly of chitosan and selenium nanoparticles for targeted delivery of CBZ. CSC has an average particle size of approximately 129 nm and a zeta potential of + 43.1 mV. It exhibited an 85 % cumulative release rate in the acidic microenvironment (pH 5.0) that simulates fungal infection sites.In vitro antifungal assays demonstrated that CSC achieved an EC₅₀ of 0.139 mg/L, showing 4.35-fold higher efficacy compared to free CBZ. Its triple-target synergistic mechanism potentially disrupts fungal cell membranes, induces oxidative stress and inhibits virulence factor synthesis, which may help mitigate resistance risks. Furthermore, CSC exhibits bidirectional vascular translocation in plants, host defense priming capacity, robust environmental stability and minimal toxicity to non-target organisms. It also promotes peanut seed germination. Enabled by green and potentially scalable preparation, CSC may reduce chemical inputs while providing a practical solution for managing vascular-colonizing pathogens such as F. oxysporum . This work aligns with the UN Sustainable Development Goals for food security and sustainable agriculture, presenting an innovative paradigm for the design of eco-friendly smart nanopesticides. • CBZ-loaded chitosan-selenium nanopesticide ( CBZ@Se − CS , CSC ): Eco-friendly Fusarium wilt control, safe, growth-promoting, low ecological impact. • pH-responsive release and bidirectional vascular translocation, boosting targeting and utilization. • The 4.35-fold higher antifungal potency than free CBZ, potentially reducing chemical fungicide dosage. • Multi-omics aids in deciphering triple-target mechanism: membrane disruption, oxidative stress, virulence suppression.
Yao et al. (Sat,) studied this question.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: