Bioluminescence Imaging of Bacterial Infections in Mice

Key Points

Bacterial infections can be monitored effectively using bioluminescence imaging techniques, enabling real-time tracking.
In this study, a luxABCDE reporter system is utilized, which produces light at the infection site, indicating metabolic activity.
Assessment using a mouse model shows the ability to non-invasively visualize and quantify infection dynamics over time.
This method may enable better understanding of chronic bacterial infections, with implications for future therapeutic interventions.

Abstract

Source: de Vries, C. R., et al., A Delayed Inoculation Model of Chronic Pseudomonas aeruginosa Wound Infection. J. Vis. Exp. (2020).This video demonstrates a bioluminescent imaging technique to monitor bacterial infection progression in a live mouse model. Pathogenic bacteria engineered with the luxABCDE reporter system emit light at the infection site, allowing visualization of metabolically active bacteria. By imaging the mouse over time, researchers can non-invasively track infection dynamics and quantify bioluminescent signal intensity.

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synapsesocial.com/papers/69a765b3badf0bb9e87da157 https://doi.org/https://doi.org/10.3791/24340

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