Oral Infection of Drosophila melanogaster with Pathogenic Bacteria

Key Points

Internal bacterial load was quantified using serial dilution and colony counting methods.
Experiment involved preparing infection vials and transferring starved flies for bacterial ingestion.
The analysis showed successful gut colonization by pathogenic bacteria in the flies observed.
This demonstrates a model system for studying bacterial infections and their effects on host organisms.

Abstract

Source: Siva-Jothy, J. A., et al. Oral Bacterial Infection and Shedding in Drosophila melanogaster. J. Vis. Exp. (2018)The video demonstrates the oral infection of Drosophila melanogaster with pathogenic bacteria. It includes the preparation of infection vials and the transfer of starved flies to enable bacterial ingestion. The procedure captures gut colonization, followed by surface sterilization and homogenization of flies. Finally, it shows quantification of internal bacterial load through serial dilution and colony counting.

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Oral Infection of Drosophila melanogaster with Pathogenic Bacteria

Key Points

Abstract

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