Metagenomic and metatranscriptomic analysis of sulfur-driven autotrophic denitrification coupled with carbon assimilation: roles of sulfur-to-nitrogen ratio and hydraulic retention time

Key Points

• Denitrification behavior shifts with variations in sulfur-to-nitrogen ratio and hydraulic retention time, indicating possible optimization strategies.
• A metagenomic and metatranscriptomic framework was utilized to assess the microbial community and gene expression related to metabolism.
• Microbial roles in denitrification processes are highlighted, showing interactions between carbon assimilation and sulfur compounds.
• Understanding these mechanisms may enable better management practices for water treatment systems focusing on nutrient reduction.