Development and validation of an LC-MS/MS method for simultaneous quantification of p-Chloroamphetamine in plasma and brain: application to pharmacokinetic and brain distribution studies in mice

Key Points

• Quantification of p-chloroamphetamine was achieved using LC-MS/MS, providing important data for pharmacokinetic analysis.
• Validation metrics included precision and accuracy in plasma and brain samples, with results supporting its reliability for future studies.
• Analysis involved observing brain distribution in mice, allowing insights into how p-chloroamphetamine is processed in the body.
• This study highlights the need for advanced profiling methods in preclinical pharmacokinetics, especially for central nervous system drugs.