ABSTRACT Metals are essential for microbial metabolism, yet their role as limiting nutrients in freshwater streams remains poorly understood. We quantified the prevalence of metal and nutrient (co‐)limitation of primary producers in 41 streams. Metal limitation was widespread with Fe limitation eliciting the strongest and most consistent biomass responses (50% of streams). Zn limitation was also common (33% of streams), marking the first evidence of Zn‐limited stream biofilms at this spatial scale. Metals were often co‐limiting with N and P, highlighting interactions between macro‐ and micronutrients. Diatoms were more responsive to Zn and cyanobacteria reached higher biomass with N and P enrichment, emphasizing divergent nutrient responses among taxa. Predictive modelling indicated that Fe and Zn limitation could be forecasted from environmental variables related to macronutrient supply. These findings challenge the long‐standing assumption that stream primary producers are rarely metal‐limited and suggest that trace metals may play an underappreciated role in regulating stream productivity, community composition and nutrient cycling.
Costello et al. (Sun,) studied this question.