Therapeutic Genome Editing In article number (e11362), Jounghyun Yoo, Seung Woo Cho, Jinmyoung Joo, and co-workers introduced a FAST-CRISPR (Fusogenic Association and Secured Transfection of CRISPR/Cas9) platform engineered for rapid cytosolic delivery of CRISPR/Cas9 ribonucleoproteins. By mediating direct membrane fusion to bypass endocytic pathways, it enables rapid cytosolic delivery and nuclear transport of ribonucleoproteins. This strategy successfully triggers targeted cancer cell apoptosis, establishing a promising non-viral genome editing tool.
Kim et al. (Sun,) studied this question.