Six novel strains (LJ205 T , TR449, ZJ450 T , ZJ454, ZJ70 T and ZJ77) were isolated from the Qinghai–Tibet Plateau, China, representing three distinct novel species. The cells were Gram-stain-positive, oxidase-negative, catalase-positive and rod-shaped. Strains LJ205 T and TR449 were strictly aerobic, whereas strains ZJ450 T , ZJ454, ZJ70 T , and ZJ77 were facultatively anaerobic. Strains LJ205 T and TR449 grew optimally at 28.0 °C, pH 9.0 and 0.5–1.0% (w/v) NaCl, whereas strains ZJ450 T , ZJ454, ZJ70 T and ZJ77 showed optimal growth at 28.0 °C, pH 7.0 and 1.0% (w/v) NaCl. Comparative analysis of 16S rRNA gene sequences revealed that all six strains belonged to the family Microbacteriaceae and represented three distinct species. Phylogenetic analysis based on 675 core genes further confirmed that the 6 isolates formed 3 well-separated clades within the family Microbacteriaceae . The DNA G+C contents of the type strains LJ205 T , ZJ450 T and ZJ70 T of the three novel species are 66.5, 66.7 and 69.1 mol%, respectively. Digital DNA–DNA hybridization and average nucleotide identity analyses confirmed that the three type strains represent distinct novel species. Strains LJ205 T and ZJ70 T contained cell-wall amino acids Ala, Glu, Gly, Lys and DAB, while ZJ450 T had Ala, Glu, Gly and Orn. Their major menaquinones were MK-13, MK-12 and MK-14 in LJ205 T and ZJ70 T , but only MK-14 and MK-13 in ZJ450 T . Polar lipids included diphosphatidylglycerol, phosphatidylglycerol and glycolipid in LJ205 T and ZJ450 T , with ZJ70 T containing an additional phospholipid. Based on genotypic, phenotypic, phylogenetic and biochemical properties, the six novel strains are grouped into three distinct lineages, each represented by a type strain, and thus constitute three novel species in the family Microbacteriaceae , for which the names Ruicaihuangia ninglii sp. nov. (type strain LJ205 T =CGMCC 1.17451 T =JCM 33727 T ), Ruicaihuangia jiafui sp. nov. (type strain ZJ450 T =GDMCC 1.1955 T =KCTC 49508 T ) and Homoserinibacter chengtaonis sp. nov. (type strain ZJ70 T =GDMCC 1.1954 T =KCTC 49509 T ) are proposed, respectively.
Luo et al. (Thu,) studied this question.
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