ABSTRACT Accurate dosing, therapeutic efficacy, patient safety, and quality control in pharmaceuticals depend on precise drug concentration monitoring. This study presents a novel fluorescent detection method employing fluorescent carbon nanoparticles (CNPs) for quantifying furosemide in pharmaceutical formulations. CNPs were synthesized via a simple one‐step hydrothermal process using N,N′‐methylenebisacrylamide (N,N‐MBA) as a precursor. Characterization by TEM, FTIR, UV–vis spectroscopy, particle size analysis, and EDX confirmed their successful formation. The synthesized CNPs exhibited strong and stable fluorescence with a quantum yield of 25.87%. Upon addition of furosemide in phosphate buffer (pH 6.5), fluorescence quenching occurred at an excitation wavelength of 320 nm and emission at 395 nm. The method displayed a linear response between fluorescence intensity and furosemide concentration (2.0–30 µg/mL) with a detection limit of 0.5 µg/mL and correlation coefficient ( R 2 ) of 0.9979. It also demonstrated excellent selectivity in the presence of common excipients. Validation confirmed high accuracy and precision. These findings highlight the potential of CNPs as a robust and sensitive platform for quality control of furosemide in pharmaceutical products.
Abdullah et al. (Sun,) studied this question.