Renal cancer is characterized by an insidious onset and a high tendency for lung metastasis in advanced stages. Here, we present a protocol for constructing an orthotopic mouse model that recapitulates the spontaneous metastasis process of human renal cell carcinoma (RCC). We describe steps for dynamically monitoring tumor growth and metastasis by in vivo bioluminescence imaging. We then detail the procedures for performing histopathological analyses. This protocol provides a reliable preclinical platform for investigating RCC metastasis mechanisms and evaluating anti-metastatic therapies. For complete details on the use and execution of this protocol, please refer to Shi et al. 1 • Protocol for constructing luciferase-expressing Renca cell lines • Surgical procedure for implanting Renca-luc cells into the mouse kidney • Guidance on using in vivo bioluminescence imaging for real-time tumor monitoring • Instructions for observing tumor pathological features via H&E staining Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. Renal cancer is characterized by an insidious onset and a high tendency for lung metastasis in advanced stages. Here, we present a protocol for constructing an orthotopic mouse model that recapitulates the spontaneous metastasis process of human renal cell carcinoma (RCC). We describe steps for dynamically monitoring tumor growth and metastasis by in vivo bioluminescence imaging. We then detail the procedures for performing histopathological analyses. This protocol provides a reliable preclinical platform for investigating RCC metastasis mechanisms and evaluating anti-metastatic therapies.
Liang et al. (Mon,) studied this question.