Protocol to generate an allo-transplantable tumor in mouse models using the YUMM1.7 cell line

Here, we present a protocol to generate an allo-transplantable tumor in mouse models using the YUMM1.7 cell line. We describe steps for mouse breeding using syngeneic C57BL/6 and allogeneic BALB/c mice, injecting the YUMM1.7 melanoma cell line into mice, and collecting and dissociating the growing tumors into single cells. We then detail the procedures for injecting the cells into mice for the generation of an allo-transplantable tumor by progressive passaging into more mismatched hosts. For complete details on the use and execution of this protocol, please refer to Rokan et al. 1 • Instructions on mouse strains and hybrids used to generate the allo-transplantable tumor • Steps for in vitro culturing of YUMM1.7 cells and the derived lines • Instructions for tumor cell transplantation and tumor dissociation into single cells • Guidance on the passaging strategy to generate the allo-transplantable tumor Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. Here, we present a protocol to generate an allo-transplantable tumor in mouse models using the YUMM1.7 cell line. We describe steps for mouse breeding using syngeneic C57BL/6 and allogeneic BALB/c mice, injecting the YUMM1.7 melanoma cell line into mice, and collecting and dissociating the growing tumors into single cells. We then detail the procedures for injecting the cells into mice for the generation of an allo-transplantable tumor by progressive passaging into more mismatched hosts.