What question did this study set out to answer?

To evaluate the efficacy of a live-attenuated vaccine in blocking SARS-CoV-2 transmission in hamsters.

March 26, 2026Open Access

Correction for Te et al., Broad beta-CoV immunity and transmission blockade by a single-dose live-attenuated vaccine with atypical codon usage

Key Points

To evaluate the efficacy of a live-attenuated vaccine in blocking SARS-CoV-2 transmission in hamsters.
Administered intranasal (I.N.) vaccination to Syrian hamsters with either a vaccine or control
Challenged vaccinated hamsters with SARS-CoV-2 and assessed exposure to naive hamsters
Monitored body weight and viral titers using oral swabs from both donor and recipient hamsters
Vaccinated hamsters exhibited reduced viral titers compared to controls
Naive hamsters showed minimal infection after 8 hours of aerosol exposure to infected donors
Direct-contact transmission was effectively blocked for 14 days post-vaccination

Abstract

A B C D cb1 vaccination blocks SARS-CoV-2 transmission in hamsters. (A) Schematic diagram for the experimental setup. 4 to 5 wk old Syrian hamsters (donors, n = 3 per group) received I. N. vaccination either with 10 5 PFU of cb1 or PBS as a control under anesthesia. On the day 21 postvaccination, all donors were challenged with 10 5 PFU of RGWT-SARS-CoV-2 or PBS. To assess aerosol transmission, nave hamsters (gray) were exposed to infected donors (yellow) placed in adjacent cages for 8 h at 24 h postinfection, then housed separately. For direct-contact transmission, donor and nave hamsters were cohoused together beginning at 24 hpi for 14 consecutive days. Body weight and infectious viral titers in oral swabs of donor (B) and recipient hamsters (C and D) were recorded. OS, Oral swab samples; CC, close contact.

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