Intestinal oxidative stress mitigation and transepithelial anti-inflammatory bioactivity mediated by EtOH-modified supercritical-CO 2 coffee pulp extract

Key Points

• The aim is to explore the antioxidant and anti-inflammatory properties of EtOH-modified CO2 coffee pulp extract post-digestion.
• Conducted in vitro simulation using INFOGEST 2.0 for gastrointestinal digestion.
• Analyzed the extract for antioxidant capacity and effects on inflammation.
• Utilized bioactivity assays to evaluate transepithelial properties.
• The extract significantly reduced oxidative stress markers in intestinal cells.
• Showed anti-inflammatory effects by lowering cytokine levels in models.
• Demonstrated potential bioactivity beneficial for gut health.