Abstract The pursuit of super-resolution imaging has produced a growing set of advanced techniques, each pushing beyond the diffraction limit in a distinct way. As these methods multiply, so do their acronyms, adding to microscopy's “alphabet soup.” This article highlights three notable approaches: minimal emission fluxes (MINFLUX), point accumulation for imaging in nanoscale topography (PAINT), and reversible saturable optical fluorescent transitions (RESOLFT). Each technique manipulates light–matter interactions differently to achieve nanoscale precision and reveal molecular details invisible to conventional microscopy. This article, Part 4 of the Alphabet Soup of Microscopy series, examines these complementary super-resolution methods.
Austin N Worden (Sun,) studied this question.