The increasing volume of organic waste, such as food and vegetable scraps, poses a significant challenge at both community and national levels. This study aimed to develop a method for decomposing household organic waste using bacteria and hydrolytic enzymes. A total of 152 bacterial isolates were screened from elephant dung, leachate, and cow manure compost. Seven isolates (A1.1, A7.2.2, A15, T12, T21.1, T36, and L11.1) exhibited hydrolytic capacity (HC ≥ 2 ± 0.1) and low antagonism (inhibition zones < 5 mm). These isolates survived at 25–37°C, pH 5–8, and in media containing 1–10 U/mL of commercial hydrolytic enzymes (amylase, protease, lipase, cellulase). Simulated waste decomposition showed that A15 (3×10 8 CFU/g), combined with cellulase (1 U/g), decomposed 1 kg of vegetable waste in 3 days. These results suggest that A15 has potential as a microbial inoculum for the decomposition of organic waste. • Household organic waste decomposition using bacteria–enzyme synergy. • Seven hydrolytic, low-antagonism isolates identified from waste sources. • Isolate A15 plus cellulase decomposed 1 kg vegetable waste in 3 days. • Selected isolates tolerated broad temperature, pH, and enzyme ranges. • A cost-effective, sustainable approach for rapid organic waste degradation.
Jirawatcharadech et al. (Sun,) studied this question.