Molecular examination of ESβL, Amp-C, and carbapenemase genes in Klebsiella pneumoniae and Escherichia coli isolates from raw milk

Objective: In this study, the detection of ESβL, Amp-c, and carbapenemase genes was aimed at K. pneumoniae and E. coli isolates obtained from raw milk collected from a total of 150 healthy cows (120 samples) located in the Ankara Region and raw milk samples (30 samples) available in markets. Materials and Methods: In this study, a total of 150 raw milk samples (120) from healthy cows and 30 from different companies were offered for sale in markets) from 9 different family businesses located in the Ankara Region were used. Results: As a result of conventional isolation from raw milk samples, 4 K. pneumoniae and 1 E. coli were identified by traditional methods and a rapid identification kit (BBL Crystal). When examined in terms of ESβL activity, CTX-M1, SHV, TEM-1, CTX-M9, OXA-1 genes; in terms of Amp-C activity, FOX gene; and terms of carbapenemase activity, OXA-48, KPC, VIM and NDM genes were detected by the classical PCR. Conclusion: A higher rate of ESβL, Amp-C ve carbapenemase genes were detected in E. coli and K. pneumonia isolates which are isolated and identified from raw milk in the Ankara Region than in many other studies. For this reason, it is considered that milk obtained from small family businesses that feed cows and raw milk sold in markets should be followed in terms of antibiotic resistance and that people who consume these products are at risk in terms of antibiotic resistance.