Transferrin (Tf) is a key protein in iron metabolism and an important biomarker for various health conditions. However, conventional immunoassay methods for Tf detection are often complex and costly. Electrochemical biosensors offer a promising alternative due to their simplicity, high sensitivity, and portability. In this study, we report the development of an electrochemical impedance biosensor for detecting human holotransferrin (holoTf) using screen‐printed carbon electrodes (SPEs) modified with Concanavalin A (ConA), a lectin that binds Tf. ConA was covalently immobilized on the SPE surface to enable the selective capture of the target protein. Electrochemical impedance spectroscopy, using ferri/ferrocyanide as a redox probe, was employed to monitor changes in electron transfer resistance associated with holoTf binding. The biosensor enabled effective, sensitive detection of holoTf, achieving a detection limit of 0.29 μM in bis‐tris propane buffer, and represents a cost‐effective potential alternative for clinical diagnostics.
Martínez et al. (Mon,) studied this question.