Objective Preeclampsia is correlated with an inflammatory condition. Necroptosis is a programmed cell death with an inflammatory state. Vitamin D has anti‐inflammatory properties; however, there has been no study linking vitamin D and necroptosis in preeclampsia. This study is aimed at evaluating vitamin D status and necroptosis activity in preeclampsia. Methods A cross‐sectional study was conducted in Jakarta during 2021–2023. Subjects were grouped into normal and preeclampsia. Following delivery, venous blood and placental samples were taken. Serum and placental 25(OH)D assays were performed by LC‐MS/MS. Immunohistochemistry was performed to measure necrosomes RIPK1, RIPK3, and MLKL in trophoblast and endothelial. Results A total of 60 subjects participated (31 normal and 29 preeclampsia). The preeclampsia group had lower gestational age (35 vs. 38 weeks), lower birth weight (3080.33 ± 454.62 g vs. 2283.27 ± 833.63 g), lower placental weight (580.40 ± 129.36 g vs. 453.06 ± 173.65 g), lower placental 25(OH)D (15.00 3.50–58.00 vs. 26.50 5.00–153.00 ng/mL, p = 0.014), and higher trophoblast RIPK3 (93.88 23.94 vs. 76.20 20.59, p = 0.003). A mild to moderate negative correlation between placental 25(OH)D and trophoblast RIPK3 (−0.352, p = 0.003), endothelial RIPK3 ( r = −0.244, p = 0.03), and trophoblast MLKL ( r = −0.296, r − 0.011) were observed. Conclusion Lower placental 25(OH)D concentration is associated with an increased placental necroptosis activity in preeclampsia.
Putri et al. (Thu,) studied this question.