Abstract Epithelial-mesenchymal transition (EMT) is an evolutionarily conserved developmental program frequently co-opted in solid tumors to drive metastasis and therapy resistance. Whether analogous transcriptional and metabolic machinery operates in blood cancers has remained unexplored. Here, we demonstrate—for the first time—that drug-tolerant persister (DTP) cells in mantle cell lymphoma (MCL), a lethal non-Hodgkin’s lymphoma, exploit an EMT-like program distinct from cancer stem cell pathways to establish stable resistance to BTK inhibitors (BTKi) and anti-CD19 CAR T-cell therapy. Using pirtobrutinib (a clinically approved non-covalent BTKi), we established a reproducible, non-stochastic DTP cell model in MCL. Integrated RNA sequencing and ultra-high-resolution metabolomics revealed that therapeutic pressure triggers a striking morphogenetic switch: proliferative lymphoma cells transform into enlarged, quiescent “Giant cells” characterized by profound dedifferentiation and loss of B-cell identity (including CD19). Upon drug withdrawal, Giant cells rapidly revert to proliferative, normal-sized progeny, exposing a previously unrecognized reversible plasticity in hematologic malignancies. Mechanistically, DTP cells rewire the TCA cycle by engaging the malate-aspartate shuttle, sustaining anabolic metabolism during drug exposure. Drug removal abruptly shifts the TCA cycle to catabolic mode, fueling re-entry into cell cycles. This metabolic switch orchestrates global transcriptomic reprogramming and elevates acetyl-CoA levels, which stabilize the core EMT transcription factor SNAI1 via non-histone protein acetylation. Remarkably, acetylated SNAI1 translocates to nucleoli, driving explosive ribosome biogenesis (marked by fibrillarin upregulation)—a hallmark less studied in conventional EMT of epithelial cancers. Perturbing ATP-citrate lyase (ACLi), SNAI1, or fibrillarin disrupts this axis: ACLi/SNAI1 blockade accelerates exit from the Giant cell state, whereas fibrillarin loss traps cells in quiescence, collectively ablating DTP plasticity and restoring therapy sensitivity. In therapy-refractory MCL patients, DTP/Giant cells can be dynamically abundant—far exceeding classic minimal residual disease—and detectable by immunohistochemistry, metabolic imaging, and single-cell RNA sequencing. Thus, unlike solid tumors where EMT primarily enables invasion, blood cancers repurpose this ancient developmental program for metabolic resilience and immune evasion. Our work establishes that an EMT-like network, orchestrated by metabolic reprogramming and nucleolar SNAI1-driven ribosome biogenesis, governs DTP cell fate in MCL. Targeting this axis—particularly ribosome biogenesis—offers a transformative strategy to eradicate persister cells and overcome resistance to BTKi and CAR T-cell therapy. Citation Format: Wei Wang, Yang Liu, Heng-Huan Lee, Fangfang Yan, Yue Fei, Yijing Li, Chengtai Yu, Lin Tan, Lorenzi Phil, Qingsong Cai, Lei Nie, Michael Wang. EMT-like reprogramming drives drug-tolerant persister cell plasticity in mantle cell lymphoma via ribosome biogenesis abstract. In: Proceedings of the American Association for Cancer Research Annual Meeting 2026; Part 1 (Regular Abstracts); 2026 Apr 17-22; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2026;86(7 Suppl):Abstract nr 392.
Wang et al. (Fri,) studied this question.