What question did this study set out to answer?

The aim is to investigate the effects of epigenetic drug treatment on histone modifications in individual tumor cells.

April 5, 2026

Abstract 3221: Profiling histone modifications in single cells to gain insight into the effects of epigenetic drug treatment on tumor cells

Key Points

The aim is to investigate the effects of epigenetic drug treatment on histone modifications in individual tumor cells.
Utilized automated, high-throughput single-cell CUT&Tag (scCUT&Tag) for histone profiling.
Assessed H3K27ac acetylation patterns in A549 lung cancer cell line.
Compared scCUT&Tag data with single-cell RNA-seq data to analyze gene expression changes.
Observed global cell-type-specific acetylation changes in response to epigenetic therapy.
Increased acetylation correlated with upregulated gene expression at promoters and enhancers.
Validated scCUT&Tag method allows deeper insight into gene regulation in tumor cells.

Abstract

Abstract Epigenomic remodeling, such as changes in chromatin state, plays a crucial role in cancer biology by regulating gene expression changes that drive tumor progression, immune evasion, and the evolution of therapeutic resistance. Popular methods to study the epigenome include assay for transposase-accessible chromatin with sequencing (ATAC-seq) and chromatin immunoprecipitation sequencing (ChIP-seq). ATAC-seq only profiles open chromatin, missing critical details about the nature of accessible regions or silenced chromatin. ChIP-seq and its newer relative, cleavage under targets and tagmentation (CUT Part 1 (Regular Abstracts); 2026 Apr 17-22; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2026;86(7 Suppl):Abstract nr 3221.

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Abstract 3221: Profiling histone modifications in single cells to gain insight into the effects of epigenetic drug treatment on tumor cells

Key Points

Abstract

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