Abstract Oncolytic HSV (oHSV) therapy is an emerging immunotherapeutic modality currently approved for melanoma in USA and EU and conditionally approved for recurrent brain tumors in Japan. Several clinical trials are investigating the utility of this approach in patients. To investigate ways to improve its therapeutic index, we analyzed the transcriptomic changes in patients with recurrent brain tumor pre and post CAN-3110 treatment (NCT03152318). Gene set enrichment analysis showed significant enrichment in the pathways of extracellular matrix (ECM) interactions and glycosaminoglycan (GAG) synthesis post virotherapy. CD44, a cell surface receptor, plays a crucial role in cell-cell and cell-ECM interactions. Apart from engaging with ECM components like hyaluronic acid (HA), collagens, fibronectin, osteopontin, matrix metalloproteinases (MMPs), etc., it also partners with several cell surface receptors like growth factor receptor family and integrins to guide cellular responses to ECM and growth factor stimulation. Here we investigated the role of CD44 signaling in regulation of both anti-viral innate immunity and anticancer adaptive immunity by creating an oHSV that encodes for the secreted extracellular portion of CD44 (OV-x44). Our results indicated that OV-x44 destabilized xCT and disrupted cellular redox homeostasis. Increased oxidative stress resulted in tumor selective STING carbonylation, and reduced TBK1 activation, thereby enhancing virus replication. On the contrary, single-cell RNA sequencing uncovered enhanced innate immune responses in immune cells with TLR7 driven activation in T cells in the tumor microenvironment (TME). To our knowledge, this is the first report to describe an oHSV that can inhibit innate intracellular anti-viral immunity in tumor cells and can also stimulate innate immune responses in the TME to guide antitumor immunity. Collectively, this study uncovers the significance of OV-x44 as a potent immune stimulating anticancer therapeutic. Citation Format: Upasana Sahu, Matthew Mullarkey, Kimberly Rivera Caraballo, Sergie Bombin, Adam Chadli, Ravindra Kolhe, James M. Markert, Bangxing Hong, Balveen Kaur. Blockade of cell-ECM interaction with an oncolytic virus suppresses tumoral STING activation while inducing antitumor immunity abstract. In: Proceedings of the American Association for Cancer Research Annual Meeting 2026; Part 1 (Regular Abstracts); 2026 Apr 17-22; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2026;86(7 Suppl):Abstract nr 7774.
Sahu et al. (Fri,) studied this question.