This work presents the development and characterization of a nickel molybdate (NiMoO 4 ) nanorod‐based electrochemical biosensor for quantitative detection of IL‐6 in blood serum samples. The NiMoO 4 nanorods (NMO) are modified with (3‐aminopropyl) triethoxysilane (APTES), followed by immobilization of IL‐6 antibodies (IL‐6 Ab) on the APTES‐NiMoO 4 /ITO working electrode via glutaraldehyde crosslinking. The fabricated IL‐6Ab/NiMoO 4 –APTES/ITO biosensor has been characterized using electrochemical techniques, scanning electron microscopy, Fourier‐transform infrared spectroscopy, X‐ray photoelectron spectroscopy, X‐ray diffraction, and Raman spectroscopy. Further, we calibrate the biosensor using standard concentrations of IL‐6 in the range from 1 to 1000 pg mL −1 and validate its performance by testing it on human blood serum samples. The biosensor exhibits 37.64 μA pg −1 mL cm −2 sensitivity with a low limit of detection of 0.02 pg mL −1 (20 fg mL −1 ) and fast detection times. We also observe stability of the sensor over 10 days, high reproducibility, and high selectivity in the presence of other interferents. The results show significant potential of the NMO nanorods‐based IL‐6 biosensor in clinical applications.
Nechikat et al. (Sun,) studied this question.