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Optical and photoacoustic imaging plays an important role in biomedical applications owing to its noninvasiveness and high resolution. Fluorescence imaging and photoacoustic imaging emerge as powerful tools to deconstruct molecular information and investigate biological processes in vivo. Despite great progress has been achieved in chemical probe synthesis, how to design probes with optimal fluorescence or photoacoustic imaging performance to dynamically visualize the biological process in vivo still faces challenges. From this perspective, we will focus on the advanced development of fluorescence and photoacoustic imaging in vivo. Furthermore, concerns and prospects for future imaging in vivo will be demonstrated.
Zhang et al. (Mon,) studied this question.