ABSTRACT Background and Aims Infections are an important cause of male infertility, yet the effects of Mycoplasma hominis on specific semen parameters remain unclear. In this meta‐analysis, we evaluated the impact of M. hominis infection on sperm quality. Methods Comprehensive searches were performed in PubMed, Embase, Web of Science, Scopus, the Cochrane Library, Google Scholar, and the Cumulative Index to Nursing and Allied Health Literature (CINAHL) from their inception through October 2025. Standardized mean differences (SMDs) and 95% confidence intervals (CIs) were calculated. Egger's regression test and examination of funnel plots were applied to evaluate potential publication bias. Subgroup and meta‐regression analyses were carried out to identify possible sources of heterogeneity, and sensitivity analysis was performed to verify the robustness of the overall estimates. Results Across 11 eligible studies, our analyses demonstrated that infertile men harboring M. hominis presented significantly lower sperm concentration (SMD = −0.815; 95% CI: −1.314 to −0.317; p = 0.001), progressive motility (SMD = −0.360; 95% CI: −0.683 to −0.037; p = 0.03), sperm viability (SMD = −0.831; 95% CI: −1.410 to −0.253; p = 0.005), and normal morphology (SMD = −0.631; 95% CI: −1.178 to −0.083; p = 0.02) compared to uninfected patients. Conversely, seminal fluid pH was consistently higher among infected subjects (SMD = 0.586; 95% CI: 0.167 to 1.006; p = 0.006). Subgroup and meta‐regression analysis suggested that study geographic location and diagnostic technique contributed to the observed heterogeneity. However, no statistically significant publication bias was detected based on Egger's test ( p = 0.10), and sensitivity analysis confirmed the robustness of the results. Conclusion Collectively, these findings support a potential relationship between M. hominis infection and deteriorated semen quality. More rigorously designed prospective studies are needed to clarify causality and enhance diagnostic guidelines.
Boujemaa et al. (Wed,) studied this question.