This study protocol investigates the effectiveness of a combined biologic approach for bone regeneration in critical-sized mandibular defects using a rabbit model. Craniomaxillofacial bone defects often require surgical intervention, as natural healing is insufficient. While autografts remain the gold standard, their limitations have driven interest in alternative regenerative strategies, particularly those involving growth factors and biomaterials. Recombinant human bone morphogenetic protein-2 (rhBMP-2) is a potent osteoinductive agent that promotes mesenchymal cell migration and osteoblastic differentiation. When delivered via a collagen scaffold, it provides a supportive matrix for controlled release and bone formation.Human amnion-chorion membrane (HACM) is a biologically active material rich in extracellular matrix components, cytokines, and growth factors, with anti-inflammatory and regenerative properties. Previous studies suggest its potential to enhance osteogenesis and tissue repair. This study aims to evaluate the synergistic effect of HACM particulates combined with rhBMP-2-loaded collagen patches on bone regeneration.Fifty rabbits will be divided into five groups, including controls and experimental combinations. Standardized mandibular defects will be surgically created and treated accordingly. Outcomes will be assessed at 2 and 4 weeks using histological, histomorphometric, and biochemical analyses, including ELISA for bone markers. The study hypothesizes that the combined treatment will significantly enhance bone formation, density, and quality compared to individual therapies, supporting its potential as an advanced regenerative strategy.
rosol dawod (Thu,) studied this question.