What question did this study set out to answer?

This research aims to identify target genes influenced by miR-318 in female Bactrocera dorsalis.

April 22, 2026Open Access

Transcriptome sequencing of Bactrocera dorsalis female adults after mimic-318 treatment

Key Points

This research aims to identify target genes influenced by miR-318 in female Bactrocera dorsalis.
Transcriptome libraries created from females treated with mimic-318
Total RNA extracted and assessed for quality
Sequencing performed on an Illumina NovaSeq platform
Sequencing yielded 150-bp paired-end reads
Quality RNA samples allowed for accurate library preparation
Identified genes potentially regulated by miR-318

Abstract

To identify potential target genes regulated by miR-318 in Bactrocera dorsalis, transcriptome libraries were constructed from female adults injected with 50 pmol mimic-318 on day 3 post-eclosion. Total RNA was extracted 24 h post-injection using the method described in the RNA and DNA isolation section. RNA concentration and purity were determined using a NanoDrop 2000 spectrophotometer (Thermo Scientific), and RNA integrity was evaluated with the RNA Nano6000 Assay Kit on an Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA). Sequencing libraries were prepared from 1 ug total RNA per sample using the Hieff NGS Ultima Dual-mode mRNA Library Prep Kit for Illumina (Yeasen Biotechnology, Shanghai, China), and sequenced on an Illumina NovaSeq platform to generate 150-bp paired-end reads, with a sequencing depth of 6 Gb per sample.

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