Self-Assembly Regulation, Drug Release Behavior, Anti-Multidrug Resistance of Redox-Responsive Gemcitabine-Quinine Nanoassemblies in Glioblastoma Therapy.

Key Points

• The aim is to evaluate the effectiveness of redox-responsive gemcitabine-quinine nanoassemblies in overcoming multidrug resistance in glioblastoma treatment.
• Evaluated drug release behavior and toxicity of GQ-S NPs in U251 cells.
• Assessed apoptotic effects and P-gp expression suppression in treated cells.
• Measured intracellular gemcitabine levels to determine accumulation effects.
• GQ-S NPs reduced P-gp expression and increased intracellular gemcitabine levels significantly.
• GQ-S NPs showed lower cytotoxicity compared to free gemcitabine and conventional nanoassemblies.
• The design achieved a substantial improvement in targeted therapy due to its low toxicity and high stability.