Abstract Background: Bacteria have developed techniques to withstand the majority of today’s medicines. Leeches are considered as a potential treatment for diseases management for their capacity to prevent blood clotting while sucking, keep it in a liquid state for prolonged storage, and displace antibacterial activities. More study is needed to understand the biology of leeches and the chemical substrates of potential treatments. Objectives: Extract Hirudo medicinalis saliva, screening antibacterial activity of crude leech saliva extract (CLSE) on test isolates ATCC bacteria ( Escherichia coli , Psedumonas auroginosa , Streptococcus pyogenes , and Staphylococcus aureus ) by disc diffusion test. Screening antibacterial activity of silver nanoparticles (Ag-NPs) saliva on test isolates by agar well diffusion, minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) test. Materials and Methods: Agar well diffusion method, antibacterial activities of CLSE on ATCC bacteria and (LSE-Ag NPs), micro broth dilution method and the agar diffusion test, (MIC) and (MBC) of LSE-Ag were determined. Results: Compared to AN 30 , the CLSE had no effect whereas LSE-Ag stopped the growth of the test isolates at 15 μL, for MIC, MBC test (10 µL) AgNPs from high to low concentrations, accordingly, in comparison to positive and negative controls show significant differences with different concentrations of AgNPs for each bacterium, but there are no significant differences between the 600 µg/mL concentrations of AgNPs compared with positive control (antibiotics). Conclusions: P. aeruginosa , S. pyogenes , E. coli , and S. aureus -related infectious illnesses may be treated using LSE-Ag.
Witwit et al. (Thu,) studied this question.