Heat-Amplified Catalytic BP-CuMOF Heterojunction Nanozyme for Antibiotic-Free Treatment of Drug-Resistant Infections

Eradicating deep-seated biofilm infections remains a formidable challenge in clinical settings due to the dense extracellular polymeric substance (EPS) barrier and the hypoxic microenvironment. Accordingly, there is an urgent need to develop nanozyme systems that integrate potent antibacterial efficacy, deep tissue penetration capability, and high biosafety. Herein, a heterojunction nanocatalytic platform is constructed via the in-situ growth of a copper-based metal-organic framework (Cu-MOF) on black phosphorus (BP) nanosheets. Benefiting from the facilitated interfacial charge separation and the localized thermal effect of BP, the resulting nanozyme exhibits a high photothermal conversion efficiency of 54.2% and markedly enhanced peroxidase-like catalytic activity under near-infrared (NIR) irradiation. In the presence of trace amounts of hydrogen peroxide (H 2 O 2 ) and NIR laser exposure, BP-CuMOF demonstrates robust biocatalytic antibacterial performance, achieving antibacterial efficiencies of 99.7% against methicillin-resistant Staphylococcus aureu s (MRSA) and effective biofilm eradication in vitro . Furthermore, this nanozyme efficiently eliminates MRSA infections in wound models and significantly accelerates wound healing in vivo , while exhibiting excellent biosafety. Collectively, this work presents a photothermally enhanced heterojunction nanozyme as an efficient and safe therapeutic strategy for the treatment of deep-seated bacterial infections, offering a promising approach for MRSA management. • BP-CuMOF heterojunction nanozyme was constructed via in-situ growth, enabling efficient interfacial charge transfer and enhanced catalytic activity. • NIR-triggered photothermal effect significantly boosts peroxidase-like activity, achieving 99.7% MRSA eradication and effective biofilm disruption. • The nanozyme promotes infected wound healing in vivo with excellent biosafety, offering a promising antibiotic-free strategy for deep infections.