Rabies Virus Detection in Milk Samples: Efficiency Comparison of Nested Rt-PCR and Taqman Real-Time PCR for Intravitam Diagnosis

Key Points

• This research aims to compare the efficiency of Nested RT-PCR and TaqMan real-time PCR for detecting rabies viral RNA in milk samples from suspected rabid animals.
• Milk samples from 17 lactating animals were collected for testing.
• Nested RT-PCR and TaqMan real-time PCR were employed for rabies detection.
• Conventional techniques, including immunofluorescence on brain tissue, were used for confirmatory diagnosis.
• Rabies viral RNA detected in 2 out of 17 milk samples using Nested RT-PCR (sensitivity 54.54%).
• Rabies viral RNA detected in 4 out of 17 milk samples using TaqMan real-time PCR (sensitivity 60%).
• Results indicate a potential method for diagnosing rabies from milk samples.