What question did this study set out to answer?

This study aims to evaluate the performance of various commercial AST methods for Burkholderia cepacia complex isolates.

May 16, 2026Open Access

Is it okay to use a commercially available AST for Burkholderia cepacia complex?

Key Points

This study aims to evaluate the performance of various commercial AST methods for Burkholderia cepacia complex isolates.
Compared Vitek 2, Sensititre, and Etest AST methods to reference broth microdilution.
Included 46 reference Burkholderia cepacia complex isolates from various laboratories.
Assessed reproducibility and performance characteristics of each method.
Sensititre demonstrated competitive performance as a commercial AST option for Burkholderia cepacia complex testing.
Vitek 2 and Etest also yielded valid results but with varying efficiency.
Challenges in AST procedures were highlighted, emphasizing the need for reliable testing methods.

Abstract

ABSTRACT In the article published by K. L. Shier, G. Gonzales, D. A. G. Perez, J. R. Bao, et al. (J Clin Microbiol 64:e00484-25, 2026, https://www.doi.org/10.1128/jcm.00484-25 ), the authors evaluated the performance of the Vitek 2 (bioMérieux), Sensititre (Thermo Fisher), and Etest (bioMérieux) Antimicrobial Susceptibility Testing (AST) methods compared to reference broth microdilution for 46 reference Burkholderia cepacia complex (BCC) isolates. This study reports on several of the most widely used commercial AST systems across laboratory settings, including commercial reference laboratories, and concludes that Sensititre may be a viable commercial option when susceptibility testing is warranted. Here, we review the reproducibility and performance characteristics of various AST methods for BCC, highlight the challenges of performing AST, and discuss the future of susceptibility testing for this organism group.

Is it okay to use a commercially available AST for Burkholderia cepacia complex?

Key Points

Abstract

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