Cryo-infarction in rats resulted in a higher 24-hour survival rate (70.59%) compared to LAD ligation (38.76%) while providing a reproducible model of localized myocardial infarction.
Cryo-infarction provides a reproducible, technically simpler, and safer alternative to LAD ligation for inducing myocardial infarction in rats, with significantly lower early mortality.
Absolute Event Rate: 70.59% vs 38.76%
Ligation of the left anterior descending (LAD) coronary artery is a widely used method for inducing myocardial infarction (MI) in experimental models; however, it is associated with substantial limitations, including high mortality rates, excessive bleeding and variability in infarct size. Cryo-infarction has emerged as a promising alternative that may overcome these challenges by providing improved survival and reproducibility. In the present study, we aimed to refine the cryo-infarction method as a reliable and practical rat model for MI. Adult male Wistar rats underwent open-chest surgery under mechanical ventilation, followed by either LAD ligation or cryo-induced infarction. Model validation was performed using TTC (2,3,5-triphenyltetrazolium chloride) staining and immunofluorescence analysis, serum troponin I measurement, and electrocardiography (ECG) assessment. Infarct severity was further evaluated by echocardiography, hematoxylin, and eosin staining, and Masson's trichrome staining. Our findings demonstrate that cryo-infarction provides several advantages over conventional ligation, including simplicity, reproducibility, and reduced mortality. These results highlight the potential of cryo-infarction as a standard approach for developing robust animal models, benefiting both basic and translational cardiovascular research.
Karimi et al. (Mon,) conducted a other in Myocardial infarction (n=197). Cryo-infarction vs. Left anterior descending (LAD) coronary artery ligation was evaluated on 24-hour survival rate. Cryo-infarction in rats resulted in a higher 24-hour survival rate (70.59%) compared to LAD ligation (38.76%) while providing a reproducible model of localized myocardial infarction.