Background Sepsis lacks reliable biomarkers for early diagnosis and treatment. This study integrates systems biology approaches, weighted gene coexpression network analysis (WGCNA), and experimental validation to identify novel diagnostic and therapeutic targets. Methods Four sepsis‐related GEO datasets were analyzed to identify differentially expressed genes (DEGs) and coexpression modules. Machine learning algorithms screened candidate biomarkers from the intersection of DEGs and module hub genes, validated via ROC analysis and immune infiltration assessment. The biological function of the top candidate was verified in vitro using LPS‐stimulated THP‐1 cells. Results GALNT14 was identified as a robust diagnostic biomarker (AUC = 0.79), showing significant correlation with neutrophil and monocyte infiltration. In vitro validation confirmed GALNT14 upregulation in the sepsis model. Functionally, GALNT14 knockdown significantly inhibited proinflammatory cytokines (IL‐1 β , IL‐6, and TNF‐ α ), whereas its overexpression exacerbated the inflammatory response and modulated cell apoptosis. Conclusion Through a synergistic framework of AI‐driven bioinformatics and wet‐lab verification, this study identifies GALNT14 as a promising diagnostic biomarker and therapeutic target, mechanistically linking it to the regulation of inflammatory responses in sepsis.
Zhu et al. (Thu,) studied this question.