Introduction: Vitamin A is essential for vision, immune function, and overall health. Quantification in pharmaceutical formulations is critical for ensuring dosage accuracy and efficacy. Methods: The method involves a technique in which a novel microfluidic thread-based analytical device (micro TAD) was used to rapidly quantify vitamin A in the form of a tablet dosage. The μTAD, prepared by dipping cotton thread in wax and pre-treating with trifluoroacetic acid (pH 3.5), gave a Prussian blue colour that varied with vitamin A concentration (20, 50 ppm) and reached its peak in the concentration range of 100 ppm. The ImageJ software was used to determine the colour intensity, with the LOD logging 4.22 ppm and the LOQ logging 12.79 ppm. Results: They compared the results to a validated RP-HPLC method (LOD 0.065 ppm, LOQ 0.211 ppm), and they were in close agreement. Discussion: The μTAD system provided a reliable, low-cost, and portable method for vitamin A quantification, though with higher detection limits compared to RP-HPLC. Despite slightly lower sensitivity, μTAD demonstrated robust precision and accuracy in validation tests. Conclusion: This study demonstrates that a μTAD can effectively quantify vitamin A in tablet form with accuracy comparable to RP-HPLC. Further improvements could enhance μTAD sensitivity, widening its practical application in drug quality control.
Makhija et al. (Wed,) studied this question.