ABSTRACT While several studies have explored the green production of silver nanoparticles (AgNPs), only a limited number have specifically investigated their antibacterial properties when synthesized using Ocimum gratissimum (OG) extracts as both a reducing and stabilizing agent. In this study, AgNPs were synthesized using OG extract, varying key parameters, including silver nitrate (AgNO 3 ) concentrations (0.01–0.05 M), solution pH (6.0–10.0), and OG extract‐to‐AgNO 3 ratios. The synthesis resulted in nanoparticles within the nanometric range, exhibiting favorable polydispersity index values. The antibacterial activity of AgNPs‐OG was performed against clinically relevant pathogens, including Pseudomonas aeruginosa and Klebsiella pneumoniae . Notably, the synthesized nanoparticles not only exhibited bactericidal effects but also effectively eradicated both planktonic and biofilm forms of these pathogens. Furthermore, the AgNPs‐OG formulation demonstrated efficacy against multidrug‐resistant K. pneumoniae strains, underscoring its potential application in combating infections caused by these bacterial threats.
Tosato et al. (Fri,) studied this question.