A digital microfluidic integrated SiNW array FET for amplification-free detection of extracellular vesicle-derived miRNAs

Key Points

• The research aims to develop a device for sensitive detection of extracellular vesicle-derived miRNAs without amplification.
• Utilized a digital microfluidic integrated SiNW array FET for analysis.
• Enabled low-volume EV enrichment.
• Discriminated single-base mismatches in miRNA detection.
• Successfully distinguished EV-miRNA expression between normal and M1 disease states.
• Achieved ultrasensitive detection of rare miRNAs without amplification.