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Introduction: Some dental adhesive systems require an etching step using phosphoric acid in order to prepare dentin and enamel surfaces. This treatment modifies the surface characteristics and the chemical compositions of these hard tissues. Objectives: The aim of this in vitro study was to analyze chemical and microscopic effects of acid etching with rubbing technique on dental tissues. Material and methods: Sixteen extracted teeth were obtained. Two etching techniques (with and without rubbing) were used to treat the enamel and dentin surfaces. Scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDX) were used to evaluate morphological and chemical changes of the enamel and dentin surfaces. Collected data were statistically analyzed by c 2 and Mann-Whitney rank sum tests.
Kharouf et al. (Fri,) studied this question.