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In our studies on fermentative production of sugar nucleotides and cytidine derivatives,1 ~6) we found that Triton X-100 treatment of yeast cells allowed them to phosphorylate nucleotide monophosphate (CMP)to nucleotide triphosphate (CTP) and then, convert them further to a final nucleotide derivative (CDP-choline).
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Agricultural and Biological Chemistry
Kyoto University
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Kimura et al. (Thu,) studied this question.