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A new species of Clostridium isolated from a methanogenic cellulose-enrichment culture of sewage sludge is described. The colonies produced by these bacteria were white, circular, and convex with smooth margins. The cells were straight, spindle-shaped rods, 0.6 by 3.0 μm in size. They were gram negative and nonmotile, and they formed round, terminal spores. A wide variety of carbohydrates was fermented by this mesophilic anaerobe. The major fermentation products were acetic acid, hydrogen, carbon dioxide, and ethanol. The deoxyribonucleic acid base composition was 28 mol% guanine plus cytosine. The name Clostridium saccharolyticum is proposed for this new species on the basis of its broad saccharolytic activity. The type strain of C. saccharolyticum is WM1 (= NRC 2533).
Murray et al. (Fri,) studied this question.