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// Jinyan Liu 1, * , Feng Li 1, * , Yu Ping 1 , Liping Wang 2 , Xinfeng Chen 1 , Dan Wang 1 , Ling Cao 1 , Song Zhao 3 , Bing Li 3 , Pawel Kalinski 5 , Stephen H. Thorne 5 , Bin Zhang 6 , Yi Zhang 1, 2, 4 1 Biotherapy Center, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, P.R. China 2 Department of Oncology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, P.R. China 3 Department of Thoracic surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, P.R. China 4 Engineering Key Laboratory for Cell Therapy of Henan Province, Zhengzhou, Henan, P.R. China 5 Department of Surgery, University of Pittsburgh Cancer Institute, University of Pittsburgh, Pittsburgh, PA, USA 6 Robert H. Lurie Comprehensive Cancer Center, Department of Medicine-Division of Hematology/Oncology, Northwestern University Feinberg School of Medicine, Chicago, IL, USA * These authors have contributed equally to this work Correspondence to: Yi Zhang, e-mail: yizhang@zzu.edu.cn Keywords: esophageal squamous cell carcinoma, T lymphocyte, chemotaxis, CCL5, CXCL10 Received: April 01, 2015 Accepted: July 06, 2015 Published: July 16, 2015 ABSTRACT Esophageal squamous cell carcinoma (ESCC) is a very common malignant tumor with poor prognosis in China. Chemokines secreted by tumors are pivotal for the accumulation of CD8 + T lymphocytes within malignant lesions in several types of cancers, but the exact mechanism underlying CD8 + T lymphocyte homing is still unknown in ESCC. In this study, we revealed that, compared with marginal tissues, the expression of both chemokine (C-C motif) ligand 5 (CCL5) and (C-X-C motif) ligand 10 (CXCL10) was upregulated in ESCC tissues. CCL5 expression was positively associated with the overall survival of patients. Meanwhile, RT-PCR data showed that the expression of CCL5 and CXCL10 was positively correlated with the local expressions of the CD8 + T lymphocyte markers ( CD8 and Granzyme B ) in tumor tissues. Correspondingly, CD8 + T lymphocytes were more frequently CCR5- and CXCR3-positive in tumor than in peripheral blood. Transwell analysis showed both CCL5 and CXCL10 were important for the chemotactic movement of CD8 + T lymphocytes. Our data indicate that CCL5 and CXCL10 serve as the key chemokines to recruit CD8 + T lymphocytes into ESCC tissue and may play a role in patient survival.
Liu et al. (Thu,) studied this question.