A demethylation-activated fluorescent DNA aptamer strategy for visualising DNA alkylation repair in living cells

Key Points

• To investigate the role of DNA demethylases in epigenetic regulation and genomic maintenance using a new fluorescent strategy.
• Developed a fluorescent DNA aptamer activated by demethylation.
• Utilized imaging techniques to analyze spatiotemporal dynamics in living cells.
• Compared signals to assess accuracy and frequency of false positives.
• Found that the new strategy accurately visualizes DNA alkylation repair with significantly reduced false positives.
• Achieved consistent signal detection across various cell types, improving upon existing methods.