High-resolution mass spectrometry-based proteomics quantified over 10,700 proteins across 16 anatomical regions and three major cardiac cell types to build a quantitative map of the healthy human heart.
Observational (n=6)
This study provides a comprehensive, high-resolution quantitative proteomic map of the healthy human heart across 16 anatomical regions and major cell types, serving as a foundational resource for cardiovascular research.
The heart is a central human organ and its diseases are the leading cause of death worldwide, but an in-depth knowledge of the identity and quantity of its constituent proteins is still lacking. Here, we determine the healthy human heart proteome by measuring 16 anatomical regions and three major cardiac cell types by high-resolution mass spectrometry-based proteomics. From low microgram sample amounts, we quantify over 10,700 proteins in this high dynamic range tissue. We combine copy numbers per cell with protein organellar assignments to build a model of the heart proteome at the subcellular level. Analysis of cardiac fibroblasts identifies cellular receptors as potential cell surface markers. Application of our heart map to atrial fibrillation reveals individually distinct mitochondrial dysfunctions. The heart map is available at maxqb.biochem.mpg.de as a resource for future analyses of normal heart function and disease.
Doll et al. (Tue,) conducted a observational in Healthy human heart and Atrial Fibrillation (n=6). High-resolution mass spectrometry-based proteomics quantified over 10,700 proteins across 16 anatomical regions and three major cardiac cell types to build a quantitative map of the healthy human heart.
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