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The analysis and interpretation of A/C (i) curves (net CO (2) assimilation rate, A, versus calculated substomatal CO (2) concentration, C (i) ) is dependent upon a number of underlying assumptions. The influence of the C (i) value at which the A/C (i) curve switches between the Rubisco- and electron transport-limited portions of the curve was examined on A/C (i) curve parameter estimates, as well as the effect of mesophyll CO (2) conductance (g (m) ) values on estimates of the maximum rate of Rubisco-mediated carboxylation (V (cmax) ). Based on an analysis using 19 woody species from the Pacific Northwest, significant variation occurred in the C (i) value where the Rubisco- and electron transport-limited portions of the curve intersect (C (iₜ) ), ranging from 20 Pa to 152 Pa and averaging c. 71 Pa and 37 Pa for conifer and broadleaf species, respectively. Significant effects on estimated A/C (i) parameters (e. g. V (cmax) ) may arise when preliminary estimates of C (iₜ), necessary for the multiple regression analyses, are set either too high or too low. However, when the appropriate threshold is used, a significant relationship between A/C (i) and chlorophyll fluorescence estimates of carboxylation is achieved. The use of the V (cmax) parameter to describe accurately the Rubisco activity from the A/C (i) curve analysis is also dependent upon the assumption that C (i) is approximately equal to chloroplast CO (2) concentrations (C (c) ). If leaf mesophyll conductance is low, C (c) will be much lower than C (i) and will result in an underestimation of V (cmax) from A/C (i) curves. A large range of mesophyll conductance (g (m) ) values was observed across the 19 species (0. 005+/-0. 002 to 0. 189+/-0. 011 mol m (-2) s (-1) for Tsuga heterophylla and Quercus garryana, respectively) and, on average, g (m) was 1. 9 times lower for the conifer species (0. 058+/-0. 017 mol m (-2) s (-1) for conifers versus 0. 112+/-0. 020 mol m (-2) s (-1) for broadleaves). When this mesophyll limitation was accounted for in V (cmax) estimates, considerable variation still existed between species, but the difference in V (cmax) between conifer and broadleaf species was reduced from c. 11 micromol m (-2) s (-1) to 4 micromol m (-2) s (-1). For example, A/C (i) curve estimates of V (cmax) were 31. 2+/-6. 2 and 42. 2+/-4. 4 micromol m (-2) s (-1), and A/C (c) curve estimates were 41. 2+/-7. 1 micromol m (-2) s (-1) and 45. 0+/-4. 8 micromol m (-2) s (-1), for the conifer and broadleaf species, respectively.
Daniel K. Manter (Fri,) studied this question.